INSL3 Polyklonaler Antikörper

INSL3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17997-1-AP

Synonyme

Insulin like 3, Insulin-like 3, Insulin-like 3 A chain, Insulin-like 3 B chain, Leydig insulin-like peptide

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  WB analysis using 17997-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17997-1-AP (INSL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

17997-1-AP bindet in WB, ELISA INSL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INSL3 fusion protein Ag12455
• Vollständiger Name: insulin-like 3 (Leydig cell)
• Berechnetes Molekulargewicht: 131 aa, 14 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC071706
• Gene symbol: INSL3
• Gene ID (NCBI): 3640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Insl3-deficient mice exhibit bilateral undescended testes located high in the abdominal cavity. INSL3 is presumed also to conform to a heterodimeric A-B structure of approximately 6 kDa once released from the producing cells. It is possible that both the mature form of INSL3 (6.6 kDa) and the pro-form(s) of INSL3 (12-14 kDa) are secreted from mammalian Leydig cells (PMID: 19329805).

Publikationen

Species	Application	Title
	WB	Exp Mol Pathol; Transcriptomic analysis of the HPT axis in a model of oligoasthenozoospermia induced by Adenine in rats; Authors - Nan Yang
rat	WB	Ecotoxicol Environ Saf; Identification of endocrine-disrupting chemicals targeting the genes and pathways of genital anomalies in males; Authors - Xiang Zhou