INTS3 Monoklonaler Antikörper

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• KD/KO Validated

INTS3 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G11B7

Kat-Nr. : 67060-1-Ig

Synonyme

2G11B7, C1orf193, C1orf60, Int3, Integrator complex subunit 3

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  WB analysis using 67060-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HEK-293 using 67060-1-Ig

  WB result of INTS3 antibody (67060-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS3 transfected HEK-293 cells.

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  WB analysis of 4T1 using 67060-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 67060-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INTS3 antibody (67060-1-Ig, Clone: 2G11B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, 4T1-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67060-1-Ig bindet in WB, IF/ICC, ELISA INTS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: INTS3 fusion protein Ag9917
• Vollständiger Name: integrator complex subunit 3
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 118 kDa
• GenBank-Zugangsnummer: BC025254
• Gene symbol: INTS3
• Gene ID (NCBI): 65123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.