INTS4 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

INTS4 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240579H4

Kat-Nr. : 83590-5-PBS

Synonyme

240579H4, Int4, MST093, MSTP093

---

• 

  WB analysis of HepG2 using 83590-5-RR (same clone as 83590-5-PBS)

  WB result of INTS4 antibody (83590-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS4 transfected HepG2 cells. This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83590-5-RR (same clone as 83590-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 83590-5-RR (same clone as 83590-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83590-5-RR (same clone as 83590-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug INTS4 Recombinant antibody (83590-5-RR, Clone:240579H4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00580-1

  Cytometric bead array standard curve of MP00580-1, INTS4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83590-5-PBS. Detection antibody: 83590-2-PBS. Standard: Ag9116. Range: 0.78-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 83590-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83590-5-RR against Human INTS4 were performed. The affinity constant is 0.146 nM.

---

Geprüfte Anwendungen

Produktinformation

83590-5-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA INTS4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: INTS4 fusion protein Ag9116
• Vollständiger Name: integrator complex subunit 4
• Berechnetes Molekulargewicht: 963aa,108 kDa; 505aa,57 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC009995
• Gene symbol: INTS4
• Gene ID (NCBI): 92105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).