INTS4 Rekombinanter Antikörper

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• KD/KO Validated

INTS4 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240579F7

Kat-Nr. : 83590-6-PBS

Synonyme

240579F7, Int4, MST093, MSTP093

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  WB analysis of HepG2 using 83590-6-RR (same clone as 83590-6-PBS)

  WB result of INTS4 antibody (83590-6-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS4 transfected HepG2 cells. This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

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  WB analysis using 83590-6-RR (same clone as 83590-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83590-6-RR (INTS4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83590-6-RR (same clone as 83590-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83590-6-RR (INTS4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83590-6-RR (same clone as 83590-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83590-6-RR (same clone as 83590-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83590-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83590-6-RR against Human INTS4 were performed. The affinity constant is 0.149 nM.

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Geprüfte Anwendungen

Produktinformation

83590-6-PBS bindet in WB, IHC, IF/ICC, ELISA INTS4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: INTS4 fusion protein Ag9116
• Vollständiger Name: integrator complex subunit 4
• Berechnetes Molekulargewicht: 963aa,108 kDa; 505aa,57 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC009995
• Gene symbol: INTS4
• Gene ID (NCBI): 92105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).