INTS6 Rekombinanter Antikörper

INTS6 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242099E6

Kat-Nr. : 84610-3-PBS

Synonyme

242099E6, DBI 1, DBI1, DDX26, DDX26A

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  Sandwich ELISA standard curve of MP01446-1

  Sandwich ELISA standard curve of MP01446-1, Human INTS6 Recombinant Matched Antibody Pair - PBS only. 84610-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag36146. 84610-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01446-1

  The mean INTS6 concentration was determined to be 61.44 ng/mL in DU 145 cell extract based on a 1.00 mg/mL extract load.

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  IHC staining of mouse liver using 84610-3-RR (same clone as 84610-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84610-3-RR (same clone as 84610-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84610-3-RR (same clone as 84610-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-3-RR, Clone: 242099E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 84610-3-RR (same clone as 84610-3-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug INTS6 Recombinant antibody (84610-3-RR, Clone:242099E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01446-1

  Cytometric bead array standard curve of MP01446-1, INTS6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84610-3-PBS. Detection antibody: 84610-1-PBS. Standard: Ag36146. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84610-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84610-3-RR against Human INTS6 were performed. The affinity constant is 1.71 nM.

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Geprüfte Anwendungen

Produktinformation

84610-3-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test INTS6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: INTS6 fusion protein Ag36146
• Vollständiger Name: integrator complex subunit 6
• Berechnetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC039829
• Gene symbol: INTS6
• Gene ID (NCBI): 26512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.