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INTS8 Polyklonaler Antikörper

INTS8 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18802-1-PBS

Synonyme

Protein kaonashi-1, Protein kaonashi 1, Integrator complex subunit 8, Int8, C8orf52

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using INTS8 Polyclonal antibody (18802-1-AP)

    WB analysis of MCF-7 using 18802-1-AP (same clone as 18802-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18802-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using INTS8 Polyclonal antibody (18802-1-AP)

    WB analysis of HeLa using 18802-1-AP (same clone as 18802-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18802-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using INTS8 Polyclonal antibody (18802-1-AP)

    IP experiment of HeLa using 18802-1-AP (same clone as 18802-1-PBS)

    IP result of anti-INTS8 (IP:18802-1-AP, 4ug; Detection:18802-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 18802-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INTS8 Polyclonal antibody (18802-1-AP)

    IF Staining of HeLa using 18802-1-AP (same clone as 18802-1-PBS)

    Immunofluorescent analysis of Hela cells, using INTS8 antibody 18802-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18802-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

18802-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA INTS8 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name integrator complex subunit 8
Berechnetes Molekulargewicht 111 kDa
Beobachtetes Molekulargewicht 95-111 kDa
GenBank-ZugangsnummerBC136754
Gene symbol INTS8
Gene ID (NCBI) 55656
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrator complex subunit 8 (INTS8) is one of the major components of RNA polymerase II and has been demonstrated to be involved in the cleavage of small nuclear RNAs and transcriptional processes (PMID: 34880328). INTS8 was robustly increased in neurodevelopmental diseases and numerous tumors. High INTS8 expression has a tight correlation with poor prognosis in multi-cancers (PMID: 30881114). Western blot analysis detected INTS8 at an apparent molecular mass of 111 kDa.