INTS9 Polyklonaler Antikörper
INTS9 Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11657-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Magengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 6 publications below |
IP | See 2 publications below |
Produktinformation
11657-1-AP bindet in WB, IHC, IP, ELISA INTS9 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | INTS9 fusion protein Ag2226 |
Vollständiger Name | integrator complex subunit 9 |
Berechnetes Molekulargewicht | 658 aa, 74 kDa |
Beobachtetes Molekulargewicht | 74 kDa |
GenBank-Zugangsnummer | BC025267 |
Gene symbol | INTS9 |
Gene ID (NCBI) | 55756 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
INTS9, also known as Integrator Complex Subunit 9, is part of the core cleavage module consisting of INTS4/INTS9/INTS11 involved in processing small nuclear RNA (snRNA) molecules. It is a subunit of the Integrator complex, which comprises 15 subunits (including INTS6L), and plays a crucial role in the 3' end processing of snRNAs. By participating in the biogenesis of snRNAs, INTS9 contributes to the proper functioning of the spliceosome, a cellular machinery responsible for the accurate splicing of precursor messenger RNA (pre-mRNA) molecules. This process is essential for generating mature messenger RNA (mRNA), which is subsequently translated into functional proteins.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for INTS9 antibody 11657-1-AP | Protokoll herunterladen |
IHC protocol for INTS9 antibody 11657-1-AP | Protokoll herunterladenl |
IP protocol for INTS9 antibody 11657-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Commun INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module. | ||
Protein Expr Purif CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells. | ||
Mol Cell Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability | ||
Mol Cell Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function | ||
Elife Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9 |