INTS9 Polyklonaler Antikörper

INTS9 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 11657-1-AP

Synonyme

CPSF, CPSF2, CPSF2L, Int9, INTS 9



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Magengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 6 publications below
IPSee 2 publications below

Produktinformation

11657-1-AP bindet in WB, IHC, IP, ELISA INTS9 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen INTS9 fusion protein Ag2226
Vollständiger Name integrator complex subunit 9
Berechnetes Molekulargewicht 658 aa, 74 kDa
Beobachtetes Molekulargewicht 74 kDa
GenBank-ZugangsnummerBC025267
Gene symbol INTS9
Gene ID (NCBI) 55756
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INTS9, also known as Integrator Complex Subunit 9, is part of the core cleavage module consisting of INTS4/INTS9/INTS11 involved in processing small nuclear RNA (snRNA) molecules. It is a subunit of the Integrator complex, which comprises 15 subunits (including INTS6L), and plays a crucial role in the 3' end processing of snRNAs. By participating in the biogenesis of snRNAs, INTS9 contributes to the proper functioning of the spliceosome, a cellular machinery responsible for the accurate splicing of precursor messenger RNA (pre-mRNA) molecules. This process is essential for generating mature messenger RNA (mRNA), which is subsequently translated into functional proteins.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for INTS9 antibody 11657-1-APProtokoll herunterladen
IHC protocol for INTS9 antibody 11657-1-APProtokoll herunterladenl
IP protocol for INTS9 antibody 11657-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cell

Assembly mechanism of Integrator's RNA cleavage module

Authors - Kevin Sabath
humanWB

Nat Commun

INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.

Authors - Kevin Sabath
humanWB

Protein Expr Purif

CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.

Authors - David Baillat
humanWB,IP

Mol Cell

Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability

Authors - Rahul Bhowmick
humanWB,IP

Mol Cell

Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function

Authors - Min-Han Lin
humanWB

Elife

Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9

Authors - Joshua D Eaton