INVS Rekombinanter Antikörper

INVS Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250038E2

Kat-Nr. : 85768-2-RR

Synonyme

INV, Inversin, KIAA0573, Nephrocystin 2, Nephrocystin-2

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  WB analysis using 85768-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85768-2-RR (INVS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85768-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85768-2-RR against Human INVS were performed. The affinity constant is 6.36 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85768-2-RR bindet in WB, ELISA INVS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: INVS fusion protein Ag0934
• Vollständiger Name: inversin
• Berechnetes Molekulargewicht: 118 kDa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC006370
• Gene symbol: INVS
• Gene ID (NCBI): 27130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling.