IPO11 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IPO11 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14403-1-AP

Synonyme

Imp11, importin 11, IPO11, Ran binding protein 11, RanBP11

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Galerie der Validierungsdaten

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  WB analysis of human testis using 14403-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14403-1-AP (IPO11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14403-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14403-1-AP (IPO11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14403-1-AP

  IP Result of anti-IPO11 (IP:14403-1-AP, 4ug; Detection:14403-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14403-1-AP (IPO11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 14403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14403-1-AP (IPO11 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14403-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14403-1-AP (IPO11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14403-1-AP bindet in WB, IP, IHC, IF IPO11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IPO11 fusion protein Ag5659
• Vollständiger Name: importin 11
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 112 kDa
• GenBank-Zugangsnummer: BC033776
• Gene symbol: IPO11
• Gene ID (NCBI): 51194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Importins are members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transport of protein and RNA cargoes. Importin-11, is a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2.

Publikationen

Species	Application	Title
human,mouse	WB,IF	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB,IHC	Urol Oncol; Importin-11 overexpression promotes the migration, invasion, and progression of bladder cancer associated with the deregulation of CDKN1A and THBS1.; Authors - Junjie Zhao ; KD Validated
mouse	WB	Int J Med Sci; Importin-11 is Essential for Normal Embryonic Development in Mice.; Authors - Ju-Young Lee ; KO Validated
		Oncotarget; Whole-exome sequencing of muscle-invasive bladder cancer identifies recurrent copy number variation in IPO11 and prognostic significance of importin-11 overexpression on poor survival.; Authors - Junjie Zhao