IPO4 Monoklonaler Antikörper

IPO4 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F3H2

Kat-Nr. : 67549-1-PBS

Synonyme

2F3H2, Imp4b, importin 4, Importin 4b, Importin-4

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  WB analysis using 67549-1-Ig (same clone as 67549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67549-1-Ig (IPO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67549-1-Ig (same clone as 67549-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IPO4 antibody (67549-1-Ig, Clone: 2F3H2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67549-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IPO4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IPO4 fusion protein Ag2281
• Vollständiger Name: importin 4
• Berechnetes Molekulargewicht: 1081 aa, 118 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC003690
• Gene symbol: IPO4
• Gene ID (NCBI): 79711
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.