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IPO5 Monoklonaler Antikörper

IPO5 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E2B9

Kat-Nr. : 67962-1-PBS

Synonyme

1E2B9, IMB3, Imp5, importin 5, Importin subunit beta 3

Filter:
  • Western Blot (WB) analysis of various lysates using IPO5 Monoclonal antibody (67962-1-Ig)

    WB analysis using 67962-1-Ig (same clone as 67962-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67962-1-Ig (IPO5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IPO5 Monoclonal antibody (67962-1-Ig)

    IHC staining of mouse testis using 67962-1-Ig (same clone as 67962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67962-1-Ig (IPO5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IPO5 Monoclonal antibody (67962-1-Ig)

    IF Staining of A431 using 67962-1-Ig (same clone as 67962-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IPO5 antibody (67962-1-Ig, Clone: 1E2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67962-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IPO5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen IPO5 fusion protein Ag30955
Vollständiger Name importin 5
Beobachtetes Molekulargewicht124 kDa
GenBank-ZugangsnummerBC001497
Gene symbol IPO5
Gene ID (NCBI) 3843
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.