IQGAP2 Polyklonaler Antikörper

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• KD/KO Validated

IQGAP2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 55189-1-AP

Synonyme

IQGAP2

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  WB analysis using 55189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55189-1-AP (IQGAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, human peripheral blood platelets
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Pankreasgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

55189-1-AP bindet in WB, IHC, ELISA IQGAP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: IQ motif containing GTPase activating protein 2
• Berechnetes Molekulargewicht: 181 kDa
• Beobachtetes Molekulargewicht: 181 kDa, 65 kDa
• GenBank-Zugangsnummer: NM_006633
• Gene symbol: IQGAP2
• Gene ID (NCBI): 10788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	BMC Cancer; Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma; Authors - Qingqing Dai
human	WB	Biochim Biophys Acta Gen Subj; Identification of the interactome of the DP1 receptor for Prostaglandin D2: Regulation of DP1 receptor signaling and trafficking by IQGAP1.; Authors - Louis Fréchette ; KD Validated
human	WB	Cell Death Dis; Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration; Authors - Yunfeng Hu ; KD Validated