IQGAP3 Polyklonaler Antikörper

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• KD/KO Validated

IQGAP3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 25930-1-AP

Synonyme

IQGAP3

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Galerie der Validierungsdaten

• 

  WB analysis using 25930-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25930-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25930-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 25930-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 25930-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

25930-1-AP bindet in WB, IHC, ELISA IQGAP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IQGAP3 fusion protein Ag23109
• Vollständiger Name: IQ motif containing GTPase activating protein 3
• Berechnetes Molekulargewicht: 1631 aa, 185 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC131536
• Gene symbol: IQGAP3
• Gene ID (NCBI): 128239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Lett; IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer.; Authors - Yulan Zeng
human	IHC	BMC Cancer; Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma; Authors - Qingqing Dai
human	WB	Biochim Biophys Acta Gen Subj; Identification of the interactome of the DP1 receptor for Prostaglandin D2: Regulation of DP1 receptor signaling and trafficking by IQGAP1.; Authors - Louis Fréchette ; KD Validated
human	WB	iScience; Oncofetal protein IGF2BP1 regulates IQGAP3 expression to maintain stem cell potential in cancer; Authors - Khine Myint
human	WB	PeerJ; IQGAP3 in clear cell renal cell carcinoma contributes to drug resistance and genome stability; Authors - Wen Li ; KD Validated