IRAK1 Monoklonaler Antikörper

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• KD/KO Validated

IRAK1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H10A7

Kat-Nr. : 66653-1-PBS

Synonyme

IRAK, IRAK 1, IRAK1, pelle

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  WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

  WB result of IRAK1 antibody (66653-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66653-1-Ig (same clone as 66653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66653-1-Ig (same clone as 66653-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66653-1-Ig (same clone as 66653-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66653-1-PBS bindet in WB, IHC, Indirect ELISA IRAK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IRAK1 fusion protein Ag0728
• Vollständiger Name: interleukin-1 receptor-associated kinase 1
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC014963
• Gene symbol: IRAK1
• Gene ID (NCBI): 3654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)