IRAK3 Rekombinanter Antikörper

IRAK3 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240975C5

Kat-Nr. : 83876-2-PBS

Synonyme

240975C5, ASRT5, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, Interleukin-1 receptor-associated kinase 3

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  WB analysis of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 83876-2-RR (same clone as 83876-2-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 83876-2-RR (same clone as 83876-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRAK3 antibody (83876-2-RR, Clone: 240975C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83876-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83876-2-PBS against Human IRAK3 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 83876-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83876-2-RR against Human IRAK3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83876-2-PBS bindet in WB, IF/ICC, Indirect ELISA IRAK3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IRAK3 fusion protein Ag4959
• Vollständiger Name: interleukin-1 receptor-associated kinase 3
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC057800
• Gene symbol: IRAK3
• Gene ID (NCBI): 11213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).