IRAK4 Polyklonaler Antikörper

IRAK4 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18221-1-AP

Synonyme

IPD1, IRAK 4, IRAK4, NY REN 64, REN64

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 18221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18221-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18221-1-AP

  IP Result of anti-IRAK4 (IP:18221-1-AP, 4ug; Detection:18221-1-AP 1:300) with K-562 cells lysate 4000ug.

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  IHC staining of human lung cancer using 18221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

18221-1-AP bindet in WB, IP, IHC, ELISA IRAK4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRAK4 fusion protein Ag12982
• Vollständiger Name: interleukin-1 receptor-associated kinase 4
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC013316
• Gene symbol: IRAK4
• Gene ID (NCBI): 51135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Mol Neurobiol; Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment; Authors - Liyun Deng
	WB	Front Pharmacol; Euphorbia Factor L2 ameliorates the Progression of K/BxN Serum-Induced Arthritis by Blocking TLR7 Mediated IRAK4/IKKβ/IRF5 and NF-kB Signaling Pathways.; Authors - Jing Tang
sheep	WB	Mediators Inflamm; Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.; Authors - Zhongjia Jiang
	WB	Evid Based Complement Alternat Med; Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation.; Authors - Xiangbing Shu
human	WB	Int Immunopharmacol; Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization; Authors - Chenyang Lu