IRE1/ERN1 Polyklonaler Antikörper

IRE1/ERN1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28164-1-PBS

Synonyme

ERN1, IRE1; ERN1, Endoplasmic reticulum-to-nucleus signaling 1, Endoribonuclease, ERN 1

---

• 

  WB analysis using 28164-1-AP (same clone as 28164-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 28164-1-AP (IRE1; ERN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28164-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

28164-1-PBS bindet in WB, Indirect ELISA IRE1/ERN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRE1/ERN1 fusion protein Ag27375
• Vollständiger Name: endoplasmic reticulum to nucleus signaling 1
• Berechnetes Molekulargewicht: 977 aa, 110 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC130405
• Gene symbol: IRE1
• Gene ID (NCBI): 2081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)