IREB2 Polyklonaler Antikörper

IREB2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29976-1-PBS

Synonyme

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  WB analysis using 29976-1-AP (same clone as 29976-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29976-1-AP (IREB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 29976-1-AP (same clone as 29976-1-PBS)

  IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:5000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 29976-1-AP (same clone as 29976-1-PBS)

  IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:1000) with A549 cells lysate 920 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29976-1-AP (same clone as 29976-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IREB2 antibody (29976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29976-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA IREB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IREB2 fusion protein Ag32233
• Vollständiger Name: iron-responsive element binding protein 2
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC017880
• Gene symbol: IREB2
• Gene ID (NCBI): 3658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.