IRF2BP1 Polyklonaler Antikörper

IRF2BP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13698-1-PBS

Synonyme

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

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  WB analysis of HeLa using 13698-1-AP (same clone as 13698-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lymph using 13698-1-AP (same clone as 13698-1-PBS)

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13698-1-AP (same clone as 13698-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 13698-1-AP (same clone as 13698-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 13698-1-AP (same clone as 13698-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 13698-1-AP (same clone as 13698-1-PBS)

  IP result of anti-IRF2BP1 (IP:13698-1-AP, 4ug; Detection:13698-1-AP 1:500) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13698-1-AP (same clone as 13698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13698-1-AP (same clone as 13698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13698-1-AP (same clone as 13698-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13698-1-AP (same clone as 13698-1-PBS)

  Immunofluorescent analysis of Hela cells, using IRF2BP1 antibody 13698-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13698-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13698-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA IRF2BP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRF2BP1 fusion protein Ag4596
• Vollständiger Name: interferon regulatory factor 2 binding protein 1
• Berechnetes Molekulargewicht: 584 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62-72 kDa
• GenBank-Zugangsnummer: BC038222
• Gene symbol: IRF2BP1
• Gene ID (NCBI): 26145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.