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IRF2BP1 Rekombinanter Antikörper

IRF2BP1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250630H5

Kat-Nr. : 86048-1-PBS

Synonyme

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

Filter:
  • Western Blot (WB) analysis of various lysates using IRF2BP1 Recombinant monoclonal antibody (86048-1-RR)

    WB analysis using 86048-1-RR (same clone as 86048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF2BP1 Recombinant monoclonal antibody (86048-1-RR)

    WB analysis using 86048-1-RR (same clone as 86048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86048-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using IRF2BP1 Recombinant monoclonal antibody (86048-1-RR)

    IF Staining of U2OS using 86048-1-RR (same clone as 86048-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IRF2BP1 antibody (86048-1-RR, Clone: 250630H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86048-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86048-1-RR

    Affinity and Kinetic Characterization of 86048-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86048-1-RR against Human IRF2BP1 were performed. The affinity constant is 0.93 nM.

Geprüfte Anwendungen

Produktinformation

86048-1-PBS bindet in WB, IF/ICC, Indirect ELISA IRF2BP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IRF2BP1 fusion protein Ag4596
Vollständiger Name interferon regulatory factor 2 binding protein 1
Berechnetes Molekulargewicht 584 aa, 62 kDa
Beobachtetes Molekulargewicht65-70 kDa
GenBank-ZugangsnummerBC038222
Gene symbol IRF2BP1
Gene ID (NCBI) 26145
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.