IRF2BP1 Rekombinanter Antikörper

IRF2BP1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250630H5

Kat-Nr. : 86048-1-RR

Synonyme

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

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  WB analysis using 86048-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86048-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 86048-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IRF2BP1 antibody (86048-1-RR, Clone: 250630H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86048-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86048-1-RR against Human IRF2BP1 were performed. The affinity constant is 0.93 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86048-1-RR bindet in WB, IF/ICC, ELISA IRF2BP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IRF2BP1 fusion protein Ag4596
• Vollständiger Name: interferon regulatory factor 2 binding protein 1
• Berechnetes Molekulargewicht: 584 aa, 62 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC038222
• Gene symbol: IRF2BP1
• Gene ID (NCBI): 26145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.