IRF3 Monoklonaler Antikörper

IRF3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6G8

Kat-Nr. : 66670-1-PBS

Synonyme

1E6G8, interferon regulatory factor 3, IRF 3, IRF-3

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  WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66670-1-Ig (same clone as 66670-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66670-1-PBS bindet in WB, IHC, ELISA IRF3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IRF3 fusion protein Ag27223
• Vollständiger Name: interferon regulatory factor 3
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC009395
• Gene symbol: IRF3
• Gene ID (NCBI): 3661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.