IRF3 Rekombinanter Antikörper

IRF3 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H6

Kat-Nr. : 80519-1-PBS

Synonyme

2H6, interferon regulatory factor 3, IRF 3, IRF-3

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  WB analysis using 80519-1-RR (same clone as 80519-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 80519-1-RR (same clone as 80519-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80519-1-RR (same clone as 80519-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80519-1-RR (same clone as 80519-1-PBS)

  Zebrafish tissue were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 80519-1-RR (same clone as 80519-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 80519-1-RR (same clone as 80519-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80519-1-RR (same clone as 80519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80519-1-RR (same clone as 80519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 80519-1-RR (same clone as 80519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80519-1-RR (same clone as 80519-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IRF3 antibody (80519-1-RR, Clone: 2H6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80519-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IRF3 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: interferon regulatory factor 3
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC009395
• Gene symbol: IRF3
• Gene ID (NCBI): 3661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.