MUM1/IRF4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MUM1/IRF4 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 11247-2-AP

Synonyme

IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5

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Galerie der Validierungsdaten

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  WB analysis using 11247-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11247-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11247-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11247-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11247-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11247-2-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11247-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 11247-2-AP

  IP Result of anti-MUM1/IRF4 (IP:11247-2-AP, 3ug; Detection:11247-2-AP 1:500) with mouse spleen tissue lysate 4000ug.

• 

  IHC staining of human thymus using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Ramos using 11247-2-AP

  1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MUM1/IRF4 (11247-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Jurkat-Zellen, Mauslungengewebe, Maus-Thymusgewebe, Raji-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Thymusgewebe, humanes Lymphomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 5 publications below

Produktinformation

11247-2-AP bindet in WB, IP, IHC, FC, ELISA MUM1/IRF4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MUM1/IRF4 fusion protein Ag1762
• Vollständiger Name: IRF 4
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC015752
• Gene symbol: IRF4
• Gene ID (NCBI): 3662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Publikationen

Species	Application	Title
human,mouse	WB	Diabetes; Obese Skeletal Muscle-Expressed Interferon Regulatory Factor 4 Transcriptionally Regulates Mitochondrial Branched-Chain Aminotransferase Reprogramming Metabolome.; Authors - Ting Yao ; KD Validated
mouse	WB	Nanotoxicology; Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.; Authors - Jie Dong
human	IHC	Diabetes Res Clin Pract; fMaggot treatment promotes healing of diabetic foot ulcer wounds possibly by upregulating Treg levels.; Authors - Jie Zhang
mouse	IHC	Int Immunopharmacol; IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.; Authors - Meixin Chen ; KO Validated
mouse	WB,IHC	Mediators Inflamm; MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.; Authors - Aijun Jia
mouse	WB	Adv Sci (Weinh); IRF4 in Skeletal Muscle Regulates Exercise Capacity via PTG/Glycogen Pathway.; Authors - Xiaopeng Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shunbin (Verified Customer) (05-01-2023)

One of the best antibody we have used for western blotting. 1:10,000 produces a single specific band. original concentration: 440 ug/ml

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: B/T cell lines

MUM1/IRF4 Antibody Western Blot validation (1:10,000 dilution) in B/T cell lines (Cat no:11247-2-AP)