IRF4 Monoklonaler Antikörper

IRF4 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A12D5

Kat-Nr. : 66451-1-PBS

Synonyme

MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4

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  WB analysis of human spleen using 66451-1-Ig (same clone as 66451-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66451-1-Ig (same clone as 66451-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66451-1-PBS bindet in WB, IHC, Indirect ELISA IRF4 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IRF4 fusion protein Ag25203
• Vollständiger Name: IRF 4
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa
• GenBank-Zugangsnummer: BC015752
• Gene symbol: IRF4
• Gene ID (NCBI): 3662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.