IRF6 Polyklonaler Antikörper

IRF6 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12928-1-AP

Synonyme

PIT, OFC6, LPS, IRF-6, IRF 6

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  WB analysis using 12928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12928-1-AP (IRF6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12928-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12928-1-AP (IRF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 12928-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 12928-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12928-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12928-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 12928-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 12928-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HaCaT using 12928-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using IRF6 antibody (12928-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HaCaT-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Magenkrebsgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12928-1-AP bindet in WB, IHC, IF/ICC, ELISA IRF6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRF6 fusion protein Ag3623
• Vollständiger Name: interferon regulatory factor 6
• Berechnetes Molekulargewicht: 467 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53-60 kDa
• GenBank-Zugangsnummer: BC014852
• Gene symbol: IRF6
• Gene ID (NCBI): 3664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The interferon regulatory factor (IRF) family of genes directs the expression and activity of interferon and thus can strictly regulate innate immunity. Interferon regulatory factor 6 (IRF6) belongs to a family of nine transcription factors that share a highly conserved helix−turn−helix DNA-binding domain and a less conserved protein-binding domain. IRF6 regulates the balance between immature ectoderm differentiation and proliferation during craniofacial morphogenesis. IRF6 functions as a tumor suppressor gene in breast cancer, cervical cancer, nasopharyngeal carcinoma and so on.

Publikationen

Species	Application	Title
mouse	WB	Front Pharmacol; Gallic acid mitigates high-fat and high-carbohydrate diet-induced steatohepatitis by modulating the IRF6/PPARγ signaling pathway; Authors - Jiahao Qiu