IRF8 Polyklonaler Antikörper

IRF8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 18977-1-AP

Synonyme

H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8

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  WB analysis of mouse spleen using 18977-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18977-1-AP (IRF8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 18977-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18977-1-AP (IRF8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lymphoma using 18977-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18977-1-AP (IRF8 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

18977-1-AP bindet in WB, IHC, IF, ELISA IRF8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ICSBP1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: NM_002163
• Gene symbol: IRF8
• Gene ID (NCBI): 3394
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

Publikationen

Species	Application	Title
mouse	WB	J Autoimmun; TIGIT-Fc fusion protein alleviates murine lupus nephritis through the regulation of SPI-B-PAX5-XBP1 axis-mediated B-cell differentiation; Authors - Junpeng Zhao
mouse	WB,IF	Mater Sci Eng C Mater Biol Appl; The unique regulation of implant surface nanostructure on macrophages M1 polarization.; Authors - Yide He
human	IHC	Transl Lung Cancer Res; Cross-talk of pyroptosis and tumor immune landscape in lung adenocarcinoma.; Authors - Xin Wang
mouse	WB,IF	J Inflamm Res; An Experimental Animal Study: Electroacupuncture Facilitates Antiviral Immunity Against Hepatitis B Virus Through the IFN-γ/JAK/STAT Axis; Authors - Yan Yang
mouse	WB	Life Sci; Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.; Authors - Tao Yang
human	WB,IHC	Life Sci; Mechanism of berberine in treating Helicobacter pylori induced chronic atrophic gastritis through IRF8-IFN-γ signaling axis suppressing.; Authors - Tao Yang