IRF9 Rekombinanter Antikörper

IRF9 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242772F12

Kat-Nr. : 85160-2-PBS

Synonyme

242772F12, IFN-alpha-responsive transcription factor subunit, Interferon-stimulated gene factor 3 gamma, IRF 9, IRF-9

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  WB analysis using 85160-2-RR (same clone as 85160-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85160-2-RR (IRF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85160-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 85160-2-RR (same clone as 85160-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85160-2-RR (IRF9 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85160-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85160-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85160-2-RR against Human IRF9 were performed. The affinity constant is 0.136 nM.

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Geprüfte Anwendungen

Produktinformation

85160-2-PBS bindet in WB, IHC, Indirect ELISA IRF9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IRF9 fusion protein Ag5365
• Vollständiger Name: IRF 9
• Berechnetes Molekulargewicht: 393 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44-48 kDa
• GenBank-Zugangsnummer: BC035716
• Gene symbol: IRF9
• Gene ID (NCBI): 10379
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.