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IRG1 Rekombinanter Antikörper

IRG1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251531C12

Kat-Nr. : 86725-1-PBS

Synonyme

ACOD1, CAD

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using IRG1 Recombinant monoclonal antibody (86725-1-RR)

    WB analysis of RAW 264.7 using 86725-1-RR (same clone as 86725-1-PBS)

    LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86725-1-RR (IRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using IRG1 Recombinant monoclonal antibody (86725-1-RR)

    WB analysis of THP-1 using 86725-1-RR (same clone as 86725-1-PBS)

    IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 86725-1-RR (IRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86725-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using IRG1 Recombinant monoclonal antibody (86725-1-RR)

    IF Staining of RAW 264.7 using 86725-1-RR (same clone as 86725-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS treated RAW 264.7 cells using IRG1 antibody (86725-1-RR, Clone: 251531C12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86725-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86725-1-PBS bindet in WB, IF/ICC, Indirect ELISA IRG1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IRG1 fusion protein Ag28913
Vollständiger Name immunoresponsive 1 homolog (mouse)
Berechnetes Molekulargewicht 53 kDa
Beobachtetes Molekulargewicht53 kDa
GenBank-ZugangsnummerNM_001258406
Gene symbol IRG1
Gene ID (NCBI) 730249
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The host Immune-Responsive Gene 1 (IRG1; also called Acod1) is a mitochondrial enzyme induced under inflammatory conditions that produces the metabolite itaconate by decarboxylating cis-aconitate, a TCA cycle intermediate. Gene expression profiling studies of murine macrophages and microglial cells have revealed that IRG1 is highly expressed under pro-inflammatory conditions. Furthermore, IRG1 is highly expressed in the pregnant uterus during the early events leading to implantation, the specific phase of pregnancy in which high levels of inflammatory cytokines are secreted. IRG1 localizes to the mitochondria and may represent a key link between immunological and metabolic processes. IRG1 has crucial functions in embryonic implantation and neurodegeneration. Also, IRG1 promotes endotoxin tolerance by increasing A20 expression in macrophages via increased ROS production. (PMID: 25640654)