IRS1 Rekombinanter Antikörper

IRS1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250510A12

Kat-Nr. : 82730-10-PBS

Synonyme

HIRS 1, insulin receptor substrate 1, IRS 1, IRS-1

---

• 

  WB analysis using 82730-10-RR (same clone as 82730-10-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82730-10-RR (IRS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82730-10-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 82730-10-RR (same clone as 82730-10-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82730-10-RR (IRS1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82730-10-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 82730-10-RR (same clone as 82730-10-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IRS1 antibody (82730-10-RR, Clone: 250510A12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82730-10-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82730-10-RR

  Biolayer interferometry (BLl) kinetic assays of 82730-10-RR against Human IRS1 were performed. The affinity constant is 7.17 nM.

---

Geprüfte Anwendungen

Produktinformation

82730-10-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IRS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IRS1 fusion protein Ag11714
• Vollständiger Name: insulin receptor substrate 1
• Berechnetes Molekulargewicht: 1242 aa, 132 kDa
• Beobachtetes Molekulargewicht: 160-185 kDa
• GenBank-Zugangsnummer: BC053895
• Gene symbol: IRS1
• Gene ID (NCBI): 3667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.