IRS2 Polyklonaler Antikörper

IRS2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20702-1-PBS

Synonyme

---

• 

  WB analysis using 20702-1-AP (same clone as 20702-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20702-1-AP (IRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 20702-1-AP (same clone as 20702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 20702-1-AP (same clone as 20702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20702-1-PBS bindet in WB, IHC, Indirect ELISA IRS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: IRS2
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 150-185 kDa
• GenBank-Zugangsnummer: NM_003749
• Gene symbol: IRS2
• Gene ID (NCBI): 8660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The family of INS receptor substrates (IRS) consists of four proteins (IRS-1-IRS-4), which were initially characterized as typical cytosolic adaptor proteins involved in INS receptor (IR) and INS-like growth factor I receptor (IGF-IR) signaling. Impaired glucose tolerance and INS resistance, and thus type 2 diabetes were demonstrated in Irs2 knockout mice. In vivo data also indicated that he regulation of beta-cell mass involves IRS2, while IRS1 is only required for proper INS production in beta-cells. IRS2, like IRS1, has a predicted molecular weight of 137 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS-PAGE as a band of approximately 150-185 kDa.