ISG15 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ISG15 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

49

Rezensionen (2)

Kat-Nr. : 15981-1-AP

Synonyme

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

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Galerie der Validierungsdaten

• 

  WB analysis using 15981-1-AP

  Untreated and IFN alpha treated A459 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15981-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15981-1-AP

  WB result of ISG15 antibody (15981-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ISG15 transfected MCF-7 cells.

• 

  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15981-1-AP (ISG15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15981-1-AP (ISG15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15981-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15981-1-AP (ISG15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, humanes Hirngewebe, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 42 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15981-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ISG15 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ISG15 fusion protein Ag8782
• Vollständiger Name: ISG15 ubiquitin-like modifier
• Berechnetes Molekulargewicht: 165 aa, 18 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC009507
• Gene symbol: ISG15
• Gene ID (NCBI): 9636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	IF	Nat Commun; Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease.; Authors - Marta Olah
human	WB	Nat Commun; ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.; Authors - Carolina Villarroya-Beltri
human	WB,IHC,IF,CoIP	J Exp Clin Cancer Res; ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma; Authors - Tong Xu ; KD Validated
human	WB, IHC, IF	Cancer Res; ISG15 is a critical microenvironmental factor for pancreatic cancer stem cells.; Authors - Bruno Sainz
human	WB	Elife; Ribosome biogenesis restricts innate immune responses to virus infection and DNA.; Authors - Christopher Bianco

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tinne (Verified Customer) (06-27-2023)

This antibody worked really well for detecting an up regulation of ISG15 in IF. Highly recommend!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human hippocampal progenitor cells

FH

Chaohui (Verified Customer) (10-19-2021)

The antibody shows a high quality of free ISG15 and conjugated ISG15 spots

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, THP1

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