ISG15 Monoklonaler Antikörper

ISG15 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G9A2

Kat-Nr. : 68408-1-PBS

Synonyme

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

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  WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 68408-1-Ig (same clone as 68408-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68408-1-PBS bindet in WB, Indirect ELISA ISG15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ISG15 fusion protein Ag8953
• Vollständiger Name: ISG15 ubiquitin-like modifier
• Berechnetes Molekulargewicht: 165 aa, 18 kDa
• Beobachtetes Molekulargewicht: 13-17 kDa
• GenBank-Zugangsnummer: BC009507
• Gene symbol: ISG15
• Gene ID (NCBI): 9636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.