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ISG20L2 Rekombinanter Antikörper

ISG20L2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250419F5

Kat-Nr. : 85992-2-PBS

Synonyme

EC:3.1.-.-, HSD-38, Interferon-stimulated 20 kDa exonuclease-like 2

Filter:
  • Western Blot (WB) analysis of various lysates using ISG20L2 Recombinant antibody (85992-2-RR)

    WB analysis using 85992-2-RR (same clone as 85992-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85992-2-RR (ISG20L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85992-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ISG20L2 Recombinant antibody (85992-2-RR)

    IHC staining of human colon cancer using 85992-2-RR (same clone as 85992-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85992-2-RR (ISG20L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85992-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ISG20L2 Recombinant antibody (85992-2-RR)

    IF Staining of HeLa using 85992-2-RR (same clone as 85992-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ISG20L2 antibody (85992-2-RR, Clone: 250419F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85992-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ISG20L2 Recombinant antibody (85992-2-RR)

    IF Staining of HEK-293 using 85992-2-RR (same clone as 85992-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ISG20L2 antibody (85992-2-RR, Clone: 250419F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85992-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85992-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ISG20L2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ISG20L2 fusion protein Ag20026
Vollständiger Name interferon stimulated exonuclease gene 20kDa-like 2
Berechnetes Molekulargewicht 353 aa, 39 kDa
Beobachtetes Molekulargewicht39 kDa
GenBank-ZugangsnummerBC000575
Gene symbol ISG20L2
Gene ID (NCBI) 81875
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ISG20L2, also named HSD38, is a member of a vertebrate exonucleases family. ISG20L2 is composed of at least two functional domains, one at its N-terminal half allowing intracellular localization and association with the binding partners and the other at its C-terminal half bearing the 3` to 5` exoribonuclease activity. The molecular weight of ISG20L2 is 39 kDa (PMID: 18065403).