ISLR Polyklonaler Antikörper

ISLR Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24306-1-AP

Synonyme

HsT17563, Immunoglobulin superfamily containing leucine-rich repeat protein, UNQ189/PRO215

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  WB analysis using 24306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24306-1-AP (ISLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 24306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24306-1-AP (ISLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 24306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24306-1-AP (ISLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24306-1-AP (ISLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 24306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ISLR antibody (24306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of SH-SY5Y using 24306-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ISLR antibody (24306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A549 using 24306-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ISLR antibody (24306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Maus-Retina-Gewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, A549-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24306-1-AP bindet in WB, IHC, IF/ICC, ELISA ISLR und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ISLR fusion protein Ag19446
• Vollständiger Name: immunoglobulin superfamily containing leucine-rich repeat
• Berechnetes Molekulargewicht: 428 aa, 46 kDa
• Beobachtetes Molekulargewicht: 44-46 kDa
• GenBank-Zugangsnummer: BC022478
• Gene symbol: ISLR
• Gene ID (NCBI): 3671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)