ISLR2 Rekombinanter Antikörper

ISLR2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241227D6

Kat-Nr. : 84076-3-RR

Synonyme

241227D6, Immunoglobulin superfamily containing leucine-rich repeat protein 2, KIAA1465, Leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein, LINX

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  WB analysis using 84076-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84076-3-RR (ISLR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84076-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84076-3-RR (ISLR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84076-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84076-3-RR (ISLR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84076-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84076-3-RR against Human ISLR2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84076-3-RR bindet in WB, IHC, ELISA ISLR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ISLR2 fusion protein Ag20505
• Vollständiger Name: immunoglobulin superfamily containing leucine-rich repeat 2
• Berechnetes Molekulargewicht: 745 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa, 130 kDa
• GenBank-Zugangsnummer: BC152429
• Gene symbol: ISLR2
• Gene ID (NCBI): 57611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ISLR2 (immunoglobulin superfamily containing leucine rich repeat 2), also known as LINX. It is expected to be located in cell membrane and the protein is mainly expressed in brain and testis. While the first knockout mouse had impaired development of the internal capsule and no hydrocephalus, a much more recent independent knockout mouse was shown to additionally have severe hydrocephalus. Although the mechanism remains unclear, it may be related to the role of ISLR2 in reorganizing the cytoskeleton of developing neurons (PMID: 30483960). The calculated molecular weight of ISLR2 is 79 kDa, this target has phosphorylation modification and glycosylation modification. And the 130 kDa band is glycosylated (PMID: 29739947).