ISM2 Polyklonaler Antikörper

ISM2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21540-1-AP

Synonyme

Thrombospondin and AMOP domain-containing isthmin-like protein 1, TAIL1, PSEC0137, Isthmin-2, isthmin 2 homolog (zebrafish)

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  WB analysis of L02 using 21540-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21540-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 21540-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 21540-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 21540-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 21540-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 21540-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21540-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of L02 using 21540-1-AP

  Immunofluorescent analysis of L02 cells using 21540-1-AP (ISM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Mausnierengewebe, Maus-Pankreasgewebe, Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21540-1-AP bindet in WB, IHC, IF/ICC, ELISA ISM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ISM2 fusion protein Ag16187
• Vollständiger Name: isthmin 2 homolog (zebrafish)
• Berechnetes Molekulargewicht: 571 aa, 64 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 55 kDa
• GenBank-Zugangsnummer: BC101020
• Gene symbol: ISM2
• Gene ID (NCBI): 145501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.