ITCH Monoklonaler Antikörper

ITCH Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E3G9

Kat-Nr. : 67757-1-PBS

Synonyme

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

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  WB analysis using 67757-1-Ig (same clone as 67757-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67757-1-Ig (same clone as 67757-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67757-1-Ig (same clone as 67757-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67757-1-Ig (same clone as 67757-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67757-1-Ig (same clone as 67757-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67757-1-Ig (same clone as 67757-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67757-1-PBS bindet in WB, IF/ICC, Indirect ELISA ITCH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ITCH fusion protein Ag14688
• Vollständiger Name: itchy E3 ubiquitin protein ligase homolog (mouse)
• Berechnetes Molekulargewicht: 903 aa, 103 kDa
• Beobachtetes Molekulargewicht: 103 kDa, 70 kDa
• GenBank-Zugangsnummer: BC011571
• Gene symbol: ITCH
• Gene ID (NCBI): 83737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.