CD41 Polyklonaler Antikörper

CD41 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(51)

Kat-Nr. : 24552-1-AP

Synonyme

CD41a, CD41B, GP2B, GPalpha IIb, GPIIb

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  WB analysis using 24552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat blood using 24552-1-AP

  mouse spleen and rat blood were subjected to SDS PAGE followed by western blot with 24552-1-AP (CD41/Integrin alpha 2b antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 24552-1-AP

  IP result of anti-CD41/Integrin Alpha 2B (IP:24552-1-AP, 4ug; Detection:24552-1-AP 1:2000) with human plasma lysate 4000ug.

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  IHC staining of mouse spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24552-1-AP (ITGA2B Antibody) at dilution of 1:50.

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  IHC staining of human spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24552-1-AP (ITGA2B Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, humanes Blutgewebe, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 6 publications below
• IF: See 16 publications below

Produktinformation

24552-1-AP bindet in WB, IHC, IF, IP, ELISA CD41 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD41 fusion protein Ag19459
• Vollständiger Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• Berechnetes Molekulargewicht: 1039 aa, 113 kDa
• Beobachtetes Molekulargewicht: 110-125 kDa
• GenBank-Zugangsnummer: BC126442
• Gene symbol: CD41
• Gene ID (NCBI): 3674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen.

Publikationen

Species	Application	Title
mouse	IF	Blood; Platelet NLRP6 protects against microvascular thrombosis in sepsis; Authors - Huimin Jiang
human	WB	Sci Adv; Platelet-derived porous nanomotor for thrombus therapy.; Authors - Mimi Wan
mouse,human	IF	Theranostics; Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages; Authors - Xueqin Li
mouse	WB	Theranostics; Platelets camouflaged nanovehicle improved bladder cancer immunotherapy by triggering pyroptosis; Authors - Jiale Tian
human	WB	J Thromb Haemost; SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia; Authors - Jiasheng Zhang
mouse	IF	Theranostics; Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.; Authors - Xin Chen