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Integrin alpha-5/CD49e Polyklonaler Antikörper
Integrin alpha-5/CD49e Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Affe, human, Maus und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10569-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A549-Zellen, 3T3-L1-Zellen, COS-7-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe |
Erfolgreiche Detektion in IHC | humanes Plazenta-Gewebe, humanes Hautkrebsgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunhistochemie (IHC) | IHC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 30 publications below |
IHC | See 2 publications below |
IF | See 10 publications below |
Produktinformation
10569-1-AP bindet in WB, IHC, IF, ELISA Integrin alpha-5/CD49e und zeigt Reaktivität mit Affe, human, Maus
Getestete Reaktivität | Affe, human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Integrin alpha-5/CD49e fusion protein Ag0860 |
Vollständiger Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
Berechnetes Molekulargewicht | 115 kDa |
Beobachtetes Molekulargewicht | 135-150 kDa |
GenBank-Zugangsnummer | BC008786 |
Gene symbol | Integrin alpha 5 |
Gene ID (NCBI) | 3678 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Integrin alpha-5/CD49e antibody 10569-1-AP | Protokoll herunterladen |
IHC protocol for Integrin alpha-5/CD49e antibody 10569-1-AP | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Biomaterials Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment. | ||
Int J Mol Sci Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway | ||
Front Cell Dev Biol Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication. | ||
Oncotarget Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma. | ||
Sci Rep Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment. | ||
Front Oncol ITGA5 Is a Novel Oncogenic Biomarker and Correlates With Tumor Immune Microenvironment in Gliomas. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (02-04-2019) | Excellent. In WB, it gives a protein band at the expected molecular weight, and this band was decreased during adipocyte differentiation, which is consistent with those reported in literature. In IF, it labels endogenous protein at the cell surface, as that reported in literature.
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