Integrin Alpha V Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Integrin Alpha V Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (3)

Kat-Nr. : 27096-1-AP

Synonyme

ITGAV, CD51, Integrin alpha-V, Integrin alpha-V heavy chain, Integrin alpha-V light chain

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  WB analysis using 27096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27096-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 27096-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (27096-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A549-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

27096-1-AP bindet in WB, IHC, IF/ICC, ELISA Integrin Alpha V und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Alpha V fusion protein Ag25825
• Vollständiger Name: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Berechnetes Molekulargewicht: 1048 aa, 116 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC136442
• Gene symbol: Integrin alpha V
• Gene ID (NCBI): 3685
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha V, also known as CD51, is a type I transmembrane glycoprotein composed of an extracellular domain, a transmembrane region and cytoplasmic domain (PMID: 2430295; 1690718). It can form heterodimers with one of the five beta integrin subunits (beta 1, 3, 5, 6, 8) that recognize ligands containing the sequence Arg-Gly-Asp, such as vitronectin, fibronectin, and osteopontin (PMID: 37087799). Integrin alpha V mainly plays a role in extracellular matrix-mediated cell adhesion and migration, differentiation, wound healing, inflammation, tumorigenesis, proliferation, angiogenesis, and metastasis.

Publikationen

Species	Application	Title
human	WB	Theranostics; Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2; Authors - Zirong Chen
rat	WB	Aging Cell; Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice.; Authors - Can Hu ; KD Validated
human	WB	Curr Biol; The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.; Authors - Adrien Presle
human	WB,IHC	Int J Mol Sci; Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression.; Authors - Justin K Messex
human	WB	Cell Signal; C1GALT1 high expression enhances the progression of glioblastoma through the EGFR-AKT/ERK cascade; Authors - Yanting Su
human	WB	Int J Mol Sci; Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients.; Authors - Emma Plana

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laura (Verified Customer) (08-28-2025)

It worked well with the protocol provided on the website

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Oligodendrocytes

FH

Alex (Verified Customer) (03-31-2025)

Exceptional focal adhesion staining in LN229 cells. Identity of the nuclear stain is unknown. Grayscale = Itgav antibody at 1:200. 4% formaldehyde fixation (methanol-free) with 0.1% Triton X-100 permeabilisation. Blocked in 2% BSA / 0.1% Triton X-100.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Neural - LN229

Integrin Alpha V Antibody Immunofluorescence validation (1:200 dilution) in Neural - LN229 (Cat no:27096-1-AP)

FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody gave a clean band at the expected molecular weight; by IF, it could not label a specific pattern.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF