CD11c Polyklonaler Antikörper

CD11c Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(69)

Kat-Nr. : 17342-1-AP

Synonyme

CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX, Leu M5

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  WB analysis using 17342-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 17342-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17342-1-AP (ITGAX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, human plasma
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 30 publications below
• IF: See 23 publications below

Produktinformation

17342-1-AP bindet in WB, IHC, IF, ELISA CD11c und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD11c fusion protein Ag11380
• Vollständiger Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Berechnetes Molekulargewicht: 1169 aa, 129 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC038237
• Gene symbol: CD11c
• Gene ID (NCBI): 3687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Publikationen

Species	Application	Title
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
	IF	Adv Sci (Weinh); Bioactive Glycyrrhizic Acid Ionic Liquid Self-Assembled Nanomicelles for Enhanced Transdermal Delivery of Anti-Photoaging Signal Peptides; Authors - Zhuxian Wang
	IHC	Acta Pharm Sin B; Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice.; Authors - Yuyu Zhu
		Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
human	IF	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
		Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang