CD11c/Integrin Alpha X Polyklonaler Antikörper

CD11c/Integrin Alpha X Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

51

Kat-Nr. : 17342-1-AP

Synonyme

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 17342-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17342-1-AP (ITGAX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HL-60 using 17342-1-AP

  1X10^6 HL-60 cells were stained with 0.2ug CD11c/Integrin alpha X antibody (17342-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 22 publications below
• IF: See 15 publications below
• FC: See 4 publications below

Produktinformation

17342-1-AP bindet in WB, IHC, IF, FC, ELISA CD11c/Integrin Alpha X und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD11c/Integrin Alpha X fusion protein Ag11380
• Vollständiger Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Berechnetes Molekulargewicht: 1169 aa, 129 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC038237
• Gene symbol: ITGAX
• Gene ID (NCBI): 3687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Publikationen

Species	Application	Title
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
	IHC	Acta Pharm Sin B; Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice.; Authors - Yuyu Zhu
	FC	Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
human	IF	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
	IF	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng

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