ITGB1BP1 Polyklonaler Antikörper

ITGB1BP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12300-1-AP

Synonyme

Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1

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  WB analysis of PC-3 using 12300-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12300-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12300-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 12300-1-AP

  IP result of anti-ITGB1BP1 (IP:12300-1-AP, 4ug; Detection:12300-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human heart using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 12300-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, humanes Hirngewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12300-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ITGB1BP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITGB1BP1 fusion protein Ag2945
• Vollständiger Name: integrin beta 1 binding protein 1
• Berechnetes Molekulargewicht: 200 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC012264
• Gene symbol: ITGB1BP1
• Gene ID (NCBI): 9270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGB1BP1, also named as ICAP1, is a key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail. It plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. ITGB1BP1 promotes transcriptional activity of the MYC promoter.