Centromere protein R Polyklonaler Antikörper

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• KD/KO Validated

Centromere protein R Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 10743-1-AP

Synonyme

ITGB3BP, HSU37139, CENP-R, CENPR, CENP R

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  WB analysis of K-562 using 10743-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10743-1-AP (Centromere protein R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10743-1-AP

  IP result of anti-Centromere protein R (IP:10743-1-AP, 4ug; Detection:10743-1-AP 1:300) with K-562 cells lysate 2400 ug.

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  IHC staining of human skin cancer using 10743-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10743-1-AP (Centromere protein R antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 10743-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Centromere protein R antibody (10743-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 4 publications below
• IP: See 3 publications below

Produktinformation

10743-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Centromere protein R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Centromere protein R fusion protein Ag1204
• Vollständiger Name: integrin beta 3 binding protein (beta3-endonexin)
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC009929
• Gene symbol: ITGB3BP
• Gene ID (NCBI): 23421
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGB3BP, also named as CENPR and NRIF3; is a transcription coregulator that can have both coactivator and corepressor functions. ITGB3BP acts as a coactivator for estrogen receptor alpha. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. ITGB3BP induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. ITGB3BP has five isoforms with MW 7 kDa, 13 kDa and 19-25 kDa.

Publikationen

Species	Application	Title
human	WB,IF,IP	Cancer Res; Identification of a novel pathway that selectively modulates apoptosis of breast cancer cells.; Authors - Tinnikov Alexander A AA
human	IF,WB,IP	J Mol Cell Biol; Phosphorylation of CENP-R by Aurora B regulates kinetochore-microtubule attachment for accurate chromosome segregation; Authors - Divine Mensah ; KD Validated
human	WB	Oncogene; Serine 28 phosphorylation of NRIF3 confers its co-activator function for estrogen receptor-alpha transactivation.; Authors - Talukder A H AH
human	WB	Stem Cells Transl Med; LncRNA NR_045147 modulates osteogenic differentiation and migration in PDLSCs via ITGB3BP degradation and mitochondrial dysfunction; Authors - Lujue Long
human	IF	J Cell Biol; Interactions with multiple inner kinetochore proteins determine mitotic localization of FACT; Authors - Julia Schweighofer
human	WB,IP,IF	Cell Rep; Condensation-dependent multivalent interactions of EB1 and CENP-R regulate chromosome oscillations in mitosis; Authors - Chengcheng Hu