Integrin Beta 4 Polyklonaler Antikörper

Integrin Beta 4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 25277-1-AP

Synonyme

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 25277-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 25277-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of mouse pancreas using 25277-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IHC staining of human breast cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 25277-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Integrin Beta 4 antibody (25277-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Maus-Pankreasgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25277-1-AP bindet in WB, IHC, IF, ELISA Integrin Beta 4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Beta 4 fusion protein Ag16460
• Vollständiger Name: integrin, beta 4
• Berechnetes Molekulargewicht: 1752 aa, 195 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC126411
• Gene symbol: ITGB4
• Gene ID (NCBI): 3691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; A mutation found in esophageal cancer alters integrin β4 mRNA splicing.; Authors - Gabriel T Kelly

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