Integrin Beta 7 Polyklonaler Antikörper

Integrin Beta 7 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11328-1-AP

Synonyme

Integrin beta 7, integrin, beta 7, ITGB7

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Galerie der Validierungsdaten

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  WB analysis of THP-1 using 11328-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11328-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11328-1-AP (ITGB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11328-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11328-1-AP (ITGB7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11328-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, humanes Lungengewebe, K-562-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

11328-1-AP bindet in WB, IHC, IF, ELISA Integrin Beta 7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Beta 7 fusion protein Ag1871
• Vollständiger Name: integrin, beta 7
• Berechnetes Molekulargewicht: 798 aa, 87 kDa
• Beobachtetes Molekulargewicht: 87-100 kDa
• GenBank-Zugangsnummer: BC015916
• Gene symbol: ITGB7
• Gene ID (NCBI): 3695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGB7 (integrin, beta7) is a single-pass type I membrane protein that belongs to the integrin beta chain family. The integrins are a large family of cell-surface glycoprotein receptors that play key roles in leucocyte adhesion, signalling, proliferation and migration by binding as heterodimers to specific ligands. The beta7 integrin associates with alpha4 integrin to form a heterodimer, alpha4/beta7, which interacts with MADCAM1, VCAM, fibronectin, and may also interact with HIV-1 gp120. The beta7 integrin can also form a heterodimer with alphaE integrin, and the dimer, alphaE/beta7 integrin, is a receptor for E-cadherin. ITGB7 is expressed in a variety of leukocyte lines.

Publikationen

Species	Application	Title
human	IHC	Hum Reprod; Expression of adhesion, attachment and invasion markers in eutopic and ectopic endometrium: a link to the aetiology of endometriosis.; Authors - Sundqvist J J
mouse	IF	J Biol Chem; Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on DSS-induced murine colitis.; Authors - Gilad Halpert
human	WB	Cell Commun Signal; Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis.; Authors - Tong Sun
human	IF	Cell Mol Gastroenterol Hepatol; Cholinergic anti-inflammatory pathway attenuates Acute Liver Failure through Inhibiting MAdCAM1/α4β7 -Mediated Gut-derived proinflammatory lymphocytes accumulation; Authors - Shan Fu