ITK Polyklonaler Antikörper

ITK Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21525-1-AP

Synonyme

Kinase EMT, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, IL2 inducible T cell kinase, EMT

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  WB analysis using 21525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21525-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21525-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21525-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 21525-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (21525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

21525-1-AP bindet in WB, IF/ICC, ELISA ITK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITK fusion protein Ag16132
• Vollständiger Name: IL2-inducible T-cell kinase
• Berechnetes Molekulargewicht: 620 aa, 72 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: BC109077
• Gene symbol: ITK
• Gene ID (NCBI): 3702
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Immunol; Role of Signal-Transducing Adaptor Protein-1 for T Cell Activation and Pathogenesis of Autoimmune Demyelination and Airway Inflammation; Authors - Kota Kagohashi
human	WB	Cytotherapy; Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma; Authors - Wenjing Luo