ITK Monoklonaler Antikörper

ITK Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9D8

Kat-Nr. : 67703-1-PBS

Synonyme

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

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  WB analysis of PBMC using 67703-1-Ig (same clone as 67703-1-PBS)

  PBMC cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (67703-1-Ig, Clone: 1F9D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67703-1-PBS bindet in WB, IF/ICC, Indirect ELISA ITK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ITK fusion protein Ag17087
• Vollständiger Name: IL2-inducible T-cell kinase
• Berechnetes Molekulargewicht: 620 aa, 72 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC109077
• Gene symbol: ITK
• Gene ID (NCBI): 3702
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITK is a member of Tec family (BTK, ITK, Tec, BMX and RLK), which expressed in normal T-lymphocytes and T-cell associated hematopoietic malignancies and have confirmed its critical role in regulating T lymphocyte function in EBV-driven lymphoproliferative disease and immune-mediated disorders. Tec kinase family members shares similarities structure, consisting of PH domain, SH3 domain, SH2 domain and kinase domain. Loss of function mutations in ITK results in hypogammaglobulinemia and CD4+ T cell loss in humans, and the patients often present with EBV-associated B cell lymphoproliferative syndrome (PMID: 30814910, PMID: 31025232).