ITPA Polyklonaler Antikörper

ITPA Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16134-1-AP

Synonyme

C20orf37, HLC14 06 P, Inosine triphosphatase, ITPA, ITPase, OK/SW cl.9

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16134-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16134-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16134-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16134-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16134-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16134-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16134-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16134-1-AP

  IP Result of anti-ITPA (IP:16134-1-AP, 4ug; Detection:16134-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16134-1-AP (ITPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16134-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ITPA antibody 11640-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Lebergewebe, Mausherzgewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lungenkarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

16134-1-AP bindet in WB, IP, IHC, IF, ELISA ITPA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITPA fusion protein Ag9128
• Vollständiger Name: inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
• Berechnetes Molekulargewicht: 194 aa, 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC010138
• Gene symbol: ITPA
• Gene ID (NCBI): 3704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.

Publikationen

Species	Application	Title
human	WB	Biochimie; Analysis of human ITPase nucleobase specificity by site-directed mutagenesis.; Authors - Hill Amanda A
human	IHC	Oncotarget; A four-gene signature predicts survival in clear-cell renal-cell carcinoma.; Authors - Jun Dai
human	WB	Biochim Biophys Acta; Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase.; Authors - Herting Greg G