ITPKB Polyklonaler Antikörper

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ITPKB Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12816-1-PBS

Synonyme

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  WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 12816-1-AP (same clone as 12816-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12816-1-AP (same clone as 12816-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 12816-1-AP (same clone as 12816-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12816-1-AP (ITPKB Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12816-1-PBS bindet in WB, IHC, Indirect ELISA ITPKB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITPKB fusion protein Ag3456
• Vollständiger Name: inositol 1,4,5-trisphosphate 3-kinase B
• Berechnetes Molekulargewicht: 946 aa, 102 kDa
• Beobachtetes Molekulargewicht: 102-110 kDa
• GenBank-Zugangsnummer: BC015009
• Gene symbol: ITPKB
• Gene ID (NCBI): 3707
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inositol 1,4,5-trisphosphate 3-kinase isoform B (IITPKB) is also named as IP3KB and possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells(PMID:21148483).Three isoforms of IP3KB occur in mammals, which are distinguished by different molecular masses, sensitivity to Ca2+/CaM(calmodulin), intracellular distribution and tissue expression (PMID:16173920). ITPKB can be cleaved into many fragments, this antibody can recognize the N-terminal peptide fragment.(PMID:15130091)